A total of 1,064 herbarium specimens of Cruciferae present at West Virginia University herbarium (WVA), as of January 1971, were examined. These specimens represent 29 genera, 61 species, and one variety, a list of which is included in this paper. A discussion of new information resulting from this study falls into four categories: 1) eight taxa [<em>Iberis amara</em> (L.) DC., <em>Isatis tinctoria</em> L., <em>Capsella rubella</em> Reut., <em>Eruca sativa</em> Mill., <em>Descurainia sophia</em> (L.) Webb, <em>Cardamine clemnatitis</em> Shuttlew., <em>Cardamine impatiens</em> L., and <em>Sibara virginica</em> (L.) Rollins] not reported for West Virginia in “Flora of West Virginia,” Edition I (Strausbaugh and Core, 1953), 2) two taxa [<em>Erucastrum gallicum</em> (Willd.) 0. E. Schulz and <em>Descurainia pinnata</em> var. <em>brachycarpa</em> (Richards.) Fern.] previously known in West Virginia from literature, but now represented by specimens at WVA, 3) one taxon [<em>Diplotaxis muralis</em> (L.) DC.] deleted because of a previously mis-identified specimen, and 4) new county records for some infrequently collected taxa. Phytogeographic aspects of some of the taxa are considered in the discussion.