A survey of the woody plants was conducted at the Big South Fork National River and Recreation Area, a 49,776 ha (123,000 acre) natural area on the Cumberland Plateau in Tennessee and Kentucky. The annotated catalogue consists of 216 (186 native) species and lesser taxa in 114 (102 native) genera and 55 (52 native) families. One hundred thirty-three taxa are county records. Magnolia fraseri and Ilex ambigua var. ambigua, collected in Scott County, are physiographic records for the Cumberland Plateau in Tennessee. Twelve listed taxa of conservation concern were documented including two Federally Threatened species (Conradina verticillata and Spiraea virginiana). The Big South Fork National River and Recreation Area was compared to 12 other natural areas including the Obed Wild and Scenic River, Land Between the Lakes, Prentice Cooper State Forest and Wildlife Management Area, and the Great Smoky Mountain National Park. Results show that the Big South Fork National River and Recreation Area is among the most floristically rich natural areas in the Southern Appalachians.