We describe for the first time Isoetes Xaltonharvillii, the hybrid between I. engelmanniii and I. caroliniana. This diploid (2n = 22) is known from two localities in the Appalachian mountains. Like other quillwort hybrids, megaspores are intermediate in morphology between the two parents. The discovery of I. ✕altonharvillii supports the segregation of its two parents as distinct species because of the apparently sterile nature of the hybrid.