A study of the occurrence and distribution of cellular slime molds (CSM) in soils of southern Appalachian spruce-fir forests was carried out during the 1985 field season. Samples for CSM isolation were collected from four different study sites: (1) Blister Run in central West Virginia, (2) Mount Rogers in southwestern Virginia, (3) Mount Mitchell in western North Carolina, and (4) Mount Coffins in the Great Smoky Mountains National Park of western North Carolina. Eight different species of cellular slime molds were isolated, but only two of these (Polysphondylium violaceum and Dictyostelium discoideum) were recovered from all four sites. Absolute density of the recovered CSM microflora was highest for Mount Mitchell, where soil samples were collected from an area near the summit characterized by almost complete mortality of the tree stratum.