The well-known European alternate-leaved spleenwort, Asplenium ✕alternifolium is reported for the first time in the New World. It is the natural hybrid of A. septentrionale (L.) Hoffm. and A. trichomanes L. Solitary plants were found in two localities in Hardy County, West Virginia, associated with the parents and other spleenworts on rock outcrops. Confirmation of their hybrid nature was accomplished by morphological, cytological, and isozyme comparisons.