The root parasite <em>Pyrularia pubera</em> Michx. (Santalaceae) is a common endemic shrub of the southern Appalachian Mountains. Hosts of <em>Pyrularia</em> were determined by field excavations and by growing <em>Pyrularia</em> seedlings with potential host seedlings in a controlled environment. Sixty tree, shrub, and herbaceous species were parasitized, including 50 genera and 31 families. Some species were not parasitized either in the forest or under culture.