The root parasite Pyrularia pubera Michx. (Santalaceae) is a common endemic shrub of the southern Appalachian Mountains. Hosts of Pyrularia were determined by field excavations and by growing Pyrularia seedlings with potential host seedlings in a controlled environment. Sixty tree, shrub, and herbaceous species were parasitized, including 50 genera and 31 families. Some species were not parasitized either in the forest or under culture.