In vitro symbiotic seed germination of the yellow fringeless-orchid, Platanthera integra (Nuttall) Gray ex Beck, is reported. Seeds of P. integra collected from the Green Swamp (Brunswick, County, North Carolina) were inoculated with mycorrhizal fungi (Epulorhiza spp.) isolated from the root-like organs of three southeastern orchids: Platanthera ciliaris (L.) Lindley, P. integrilabia (Correll) Luer, P. integra. All fungi promoted seed germination, and leaf-bearing seedlings were obtained using the fungus from P. integrilabia. Seeds exposed to prolonged (1 and 2 hr) scarification followed by white light pretreatment (12 hr: 12 hr; L:D) yielded a higher percentage of leafless seedlings (protocorms) compared to standard techniques. We advocate the widespread preservation of existing orchids and their habitats in order to safeguard mycor- rhizal fungi for use in conservation.