Previously, four hybrids have been known to occur naturally among certain members of the “rostrate” violets of Section Viola in the genus Viola. During examinations of herbarium specimens in a systematic study of the section, three new hybrids were found from the Appalachian region, two represented by a number of specimens from widely scattered localities. The morphology of all three is described, and two, V. appalachiensis ✕ striata and V. striata ✕ walteri are given the names V. ✕wujekii hybr. nov. and V. ✕cooperrideri hybr. nov., respectively. Low pollen viability and cleistogamous seed set are discussed. This constitutes the first report of interspecific hybridization involving V. appalachiensis Henry or V. walteri House.