A floristic study of the Bear Creek Natural Area, Stewart County, Tennessee documented the presence of 733 (610 native and 123 introduced) vascular species representing 388 genera and 111 families. The four largest families, Asteraceae (85 species), Poaceae (83), Cyperaceae (44), and Fabaceae (39) account for 34 percent of the species. Eleven taxa are considered elements of concern, including two under federal review. The area is within the Tennessee Valley Authority’s (TVA) Land Between the Lakes, is a registered Tennessee natural area, a designated TVA forest study and natural area, and has been recommended for designation as a National Natural Landmark. In addition to habitat diversity and the large flora with numerous rare elements, the site is significant because it represents the rare and mostly decimated western form of the mixed mesophytic forest.