The Duke Forest (Durham and Orange counties, North Carolina), managed by Duke University’s School of Forestry and Environmental Studies, contains a diversity of forested and nonforested habitats. Vascular plants were collected from 1986 to 1989, and the Duke University Herbarium was searched, in order to obtain a vouchered flora of the Duke Forest. The flora consists of 978 species, including 169 exotics. Eighty-four species are additions to the Durham or Orange County floras, as recorded by Radford et al.