The vascular flora of Pike County, Alabama was surveyed from 1987 to 2001. A total of 1190 species and two named hybrids in 589 genera from 160 families are reported. Forty-five species represented by herbarium specimens or reported in the literature as occurring in Pike County were not re-collected during this study. Thirty-four species appear on the Inventory List of Rare, Threatened and Endangered Plants, Animals and Natural Communities of Alabama as compiled by the Alabama Natural Heritage Program. Two hundred thirty-eight of the species, or approximately 20 percent of the flora, are considered non-indigenous. Families with the largest number of taxa were Asteraceae (140), Poaceae (137), Fabaceae (86), Cyperaceae (68), Rosaceae (40), and Liliaceae (32).